The effectiveness of PCR-RT method for etiological diagnosis of atypical forms of whooping cough

The aim of the study was to evaluate effectiveness PCR-RТ method using PCR-RТ-IS test system for etiological diagnosis whooping cough in examination patients with infectious respiratory pathology prolonged and contact persons. 113 children aged from 1 month 17 years, 11 months 29 days, 146 family members were examined. Regardless initial diagnosis, all examined by bacteriological, molecular genetic serological methods. For diagnostics a commercial kit PCR-RT-IS test-system developed at Gamaleya Research Center used. persons bacteriological test-system. During diagnoses «Acute viral infectious», rhinopharyngitis», laryngotracheitis», bronchitis», «Pneumonia» established. severity different – typical spastic «whooping cough» complete absence cough. By we developed, DNA pertussis pathogen detected groups rhinopharyngitis» 34.4% cases, laryngotracheitis» 64.3%, bronchitis» 69%, 33.3% cases. In number cases combined course infections (respiratory syncytial virus, rhinovirus others) detected. causative agent 51,4%